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Experimental Protocols

This mirrors files containing experimental protocols developed at the National Institutes of Health. 

The protocols contain detailed step-by-step information on how to plan, set-up and analyze experiments.

For more specific and up-to-date information please refer to the following books, which have specific information on SEDFIT and SEDPHAT functions as side-bars nested into the description of theory and experiment of analytical ultracentrifugation (AUC):

https://www.crcpress.com/Basic-Principles-of-Analytical-Ultracentrifugation/Schuck-Zhao-Brautigam-Ghirlando/p/book/9781498751155

https://www.crcpress.com/Sedimentation-Velocity-Analytical-Ultracentrifugation-Discrete-Species/Schuck/p/book/9781498768948

https://www.crcpress.com/Sedimentation-Velocity-Analytical-Ultracentrifugation-Interacting-Systems/Schuck-Zhao/p/book/9781138035287

You can also consult the step-by-step protocol https://www.nature.com/articles/nprot.2017.064 (this is using fluorescence optical data, but the data analysis applies equally to other optical systems).

Sedimentation Equilibrium

Sedimentation Velocity

Flow Chart for AUC Experiments

Criteria for Buffer Selections

Grid for SV and SE Run Conditions when Using Different Optical Systems

Typical Strategies for Common Problems