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Tutorials

This new section is planned to be extended with new step-by-step tutorials. 

Sedimentation Equilibrium for Protein-DNA interaction

Isothermal Titration Calorimetry Analysis Example

Tutorial for dealing with 6-channel centerpieces in SEDPHAT (powerpoint presentation and data)

The following is recapitulating the general concepts for using SEDPHAT:  Tutorial for Getting Started

For more recent information on sedimentation velocity analysis of interacting systems, you can also consult the step-by-step protocol https://www.nature.com/articles/nprot.2017.064 (this is using fluorescence optical data, but the data analysis applies equally to other optical systems).

Also, note that a general introduction to the study of protein interactions by analytical ultracentrifugation can be found at the website of the PBR/DBEPS at NIH.  This includes an introduction to the general principles of AUC and detailed experimental protocols.